Determination of intracellular glutathione and glutathione disulfide using high performance liquid chromatography with acidic potassium permanganate
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چکیده
This is the published version: McDermott, Geoffrey P., Francis, Paul S., Holt, Kayla J., Scott, Kristen L., Martin, Sheree D., Stupka, Nicole, Barnett, Neil W. and Conlan, Xavier A. 2011, Determination of intracellular glutathione and glutathione disulfide using high performance liquid chromatography with acidic potassium permanganate chemiluminescence detection, Analyst, vol. 136, no. 12, pp. 2578-2585. Available from Deakin Research Online: http://hdl.handle.net/10536/DRO/DU:30040441
منابع مشابه
Determination of intracellular glutathione and glutathione disulfide using high performance liquid chromatography with acidic potassium permanganate chemiluminescence detection.
Measurement of glutathione (GSH) and glutathione disulfide (GSSG) is a crucial tool to assess cellular redox state. Herein we report a direct approach to determine intracellular GSH based on a rapid chromatographic separation coupled with acidic potassium permanganate chemiluminescence detection, which was extended to GSSG by incorporating thiol blocking and disulfide bond reduction. Importantl...
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Glutathione (GSH) is one of the most important antioxidants that plays an essential role in detoxification of reactive oxygen species (ROS) which oxidizes to glutathione disulfide (GSSG). Paraquat (PQ), awidely used herbicide, causes pulmonary injury with the productionof ROS. Excessive ROS accumulation as a consequence of PQ exposure are frequently targeted by GSH thereby oxidative stress lead...
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References 1. White AC, Thannickal VJ, Fanburg BL. Glutathione deficiency in human disease. J Nutr Biochem 1994;5:218–26. 2. Ubbink JB, Vermaak WJH, Bissbort S. Rapid high-performance liquid chromatographic assay for total homocysteine levels in human serum. J Chromatogr B 1991;565:441–6. 3. Araki A, Sako Y. Determination of free and total homocysteine in human plasma by high performance liquid...
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